Project Impurity

Project Impurity is a main quest in Fallout 3.

Detailed walkthrough
Once the player approaches the entrance of the Jefferson Memorial as required by the related main quest Take it Back!, this quest will activate soon after. The exact objective outlined is: "Infect the Project Purity water supply... or not".

The player can choose to infect the water supply with the Modified FEV given to him/her by President Eden once inside of the Rotunda which will complete this quest immediately after. This is done via the purifier's console just to the right of the entrance to the purifier's primary chamber.

Should the player choose to not infect the water, the quest will fail. It grants no XP upon it's completion and the modified FEV will remove itself from the player's inventory regardless of their choice in infecting the water or not.

If the player chooses to infect the water supply with the modified FEV, the player is garnered -1000 Karma points.