Lady Killer

 or Lady Killer (depending on the Player Character gender) are Fallout 3 perks.

In combat, you do +10% damage against opposite sex opponents. Outside of combat, you'll sometimes have access to unique dialogue options when dealing with the opposite sex (the latter is much like the Sex Appeal Trait). Both perks' images feature a female character who can be construed to be the female analogue of the Vault Boy.

Black Widow

 * You can talk Ronald Laren in the Nuka-Cola Challenge quest into a threesome. He then runs to the factory himself. He always seems to end up dead just inside the door to the Nuka-Cola factory if you do this, even if you accompany him all the way there; if he is still alive when the two of you arrive, he will just wait in the lobby while you explore the factory alone. When you come back to the entrance, he will be dead.
 * You can talk Burke in Megaton into paying you double to do the job, or to give up on the bomb entirely.
 * You can talk Karl (during the Blood Ties quest) into giving you the password to Ian West's room.
 * You can talk The Mechanist into stop fighting his nemesis, the AntAgonizer. You also get his costume.
 * You can talk Dukov out of his key without paying for it for the You Gotta Shoot 'Em in the Head quest.
 * You can talk Uncle Roe into organizing the trade caravans.
 * You can convince Ramsey into selling slaves to you during Into the Pitt.
 * You can talk with Defender Morrill at the beginning of Operation Anchorage, so you can come through after the elevator, but that should not be a problem even if you don't have the Black Widow perk.
 * You can convince Wernher to give you 120 5.56mm Rounds should you decide to side with the slaves during Free Labor.
 * You can use this perk on Sibley in the beginning of the Operation: Anchorage quest-line, where he will lead you to Protecter McGraw, without the aggression. He still turns on you at the end, though.
 * You can use this perk on Paulson when you first meet him during Mothership Zeta. He will tell you why he wants revenge.

Lady Killer

 * Nova in Megaton will provide you with the password to Moriarty's terminal.
 * Brianna in the Blood Ties quest will provide you with the password to Ian West's cell.
 * You can talk the AntAgonizer into stopping her attacks on the town and fighting The Mechanist. You also get her costume.
 * You can talk Fantasia into stealing Dukov's key for the You Gotta Shoot 'Em in the Head quest.
 * You can talk Agatha into giving you ammo to help you during the Agatha's Song quest.
 * In Operation: Anchorage, you can calm down Specialist Olin and get more detailed information on questions. Also, the conversation will not end after each question.
 * In The Pitt, Vikia will tell you that she's immune and will not sleep with you, regardless how good you are at sex.